Plant Tissue Culture: Theory and Practice, 2e

Authors: T. Pullaiah -M.V. Subba Rao -E. Sreedevi

Publisher: Scientific Publishers

Contents:

1. History of Plant Tissue Culture

 1.1. Introduction

 1.2. Totipotency of the Cells

 1.3. Morphogenesis

 1.4. History of Plant Tissue Culture

 1.5. Applications of Plant Tissue Culture

 1.6. Terminology

 1.7. Books for Further Reading

2. Laboratory Requirements for Plant Tissue Culture

 2.1. Laboratory Organization

3. Tissue Culture Media

 3.1. Introduction

 3.2. Media Composition

 3.3. Media Preparation

 3.4. Commonly used Concentrations in Tissue Culture

4. Methods of Sterilization

 4.1. Introduction

 4.2. Sterilization by Heat

 4.3. Filter Sterilization

 4.4. Chemicals

 4.5. Infrared Radiation

 4.6. Surfactants

 4.7. Antibiotics for Control of Bacterial and Fungal Contaminants

 4.8. Maintenance of Aseptic Conditions

 4.9. Surface Sterilization of Explant

 4.10. Transfer of An Explant

5. Cell and Suspension Culture

 5.1. Introduction

 5.2. Procedure for Cell and Suspension Culture

 5.3. Establishment of Cell Suspension Culture

 5.4. Cell Suspension Culture and Cell Growth

 5.5. Formation of Somatic Embryos and Plant Regeneration

 5.6. Types of Suspension Cultures

 5.7. Growth Patterns in Suspension Culture

 5.8. Measurement of Cell Growth in Suspension Cultures

 5.9. Synchronization of Suspension Cell Cultures

 5.10. Technique for Single Cell Culture

 5.11. Tests for Viability of Cultured Cells

 5.12. Advantages of Cell and Suspension Culture

 5.13. Plant Cell Reactors

 5.14. Immobilized Plant Cell Reactors

 5.15. Culture of Isolated Single Cells

6. Somatic Embryogenesis

 6.1. Introduction

 6.2. Some Examples of Somatic Embryogenesis

 6.3. Theories on Embryogenesis

 6.4. Factors Affecting Somatic Embryogenesis

 6.5. Anatomy and Cytology to Somatic Embryo Development

7. Synthetic Seeds

 7.1. Introduction

 7.2. Encapsulation

 7.3. Production of Artificial Seeds

 7.4. Applications

 7.5. Limitations

 7.6. Uses of Artificial Seeds

 7.7. Reference

8. Micropropagation of Higher Plants

 8.1. Introduction

 8.2. Somatic Cell Embryogenesis

 8.3. Production of Adventitious Shoots and Related Organs

 8.4. Enhanced Axillary Branching

 8.5. Steps Involved in Plant Multiplication

 8.6. Buds Culture

 8.7. Factors Affecting Micropropagation

 8.8. Micropropagation of Ornamental Plants

 8.9. Advantages of Micropropagation

9. Haploid Production

 9.1. Introduction

 9.2. Anther Culture

 9.3. Pollen Culture

 9.4. Comparison between Anther and Pollen Cultures

 9.5. Significance of Uses of Haploids

 9.6. Limitations

 9.7. Gynogenesis

 9.8. Bulbosum Technique

10. Nucellus Culture, Ovule Culture, Ovary Culture and Flower Culture

 10.1. Nucellus Culture

 10.2. Ovule Culture

 10.3. Ovary Culture

 10.4. Flower Culture

11.  Embryo and Endosperm Culture

 11.1. Embryo Culture

 11.2. Endosperm Culture

12. Protoplast Culture and Somatic Hybridization

 12.1. Introduction

 12.2. Sources of Protoplasts

 12.3. Isolation of Protoplasts

 12.4. Culture of Protoplasts

 12.5. Applications

 12.6. Protoplast Fusion and Somatic Hybridization

 12.7. Cybrids

 12.8. Applications

13. Somaclonal Variations

 13.1. Introduction

 13.2. Origin of Somaclonal Variation

 13.3. Isolation of Variants

 13.4. Gametoclonal Variation

14. Germplasm Conservation

 14.1. Introduction

 14.2. Determination of Cell Survival

 14.3. Action of Cryoprotectants

 14.4. Factors Affecting Revival of Frozen Cells

 14.5. Applications

15. Production of Secondary Metabolites Through Tissue Culture

 15.1. Introduction

 15.2. Types of Cultures

 15.3. Bioreactors

 15.4. Cell Immobilization

 15.5. Improving the Product Quality and Quantity

 15.6. Commercial Production

 15.7. Characteristics of Plant Cells in Suspension Culture

 15.8. Factors Affecting Secondary Metabolite Production in Cell 
  Suspension Cultures

 15.9. Ways to Increase Product Formation

16. Isolation of Mutants From Cell Culture

 16.1. Introduction

17. Practicals

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