Plant Tissue Culture: Theory and Practice, 2e
Authors: T. Pullaiah -M.V. Subba Rao -E. Sreedevi
Publisher: Scientific Publishers
Contents:
1. History of Plant Tissue Culture
1.1. Introduction
1.2. Totipotency of the Cells
1.3. Morphogenesis
1.4. History of Plant Tissue Culture
1.5. Applications of Plant Tissue Culture
1.6. Terminology
1.7. Books for Further Reading
2. Laboratory Requirements for Plant Tissue Culture
2.1. Laboratory Organization
3. Tissue Culture Media
3.1. Introduction
3.2. Media Composition
3.3. Media Preparation
3.4. Commonly used Concentrations in Tissue Culture
4. Methods of Sterilization
4.1. Introduction
4.2. Sterilization by Heat
4.3. Filter Sterilization
4.4. Chemicals
4.5. Infrared Radiation
4.6. Surfactants
4.7. Antibiotics for Control of Bacterial and Fungal Contaminants
4.8. Maintenance of Aseptic Conditions
4.9. Surface Sterilization of Explant
4.10. Transfer of An Explant
5. Cell and Suspension Culture
5.1. Introduction
5.2. Procedure for Cell and Suspension Culture
5.3. Establishment of Cell Suspension Culture
5.4. Cell Suspension Culture and Cell Growth
5.5. Formation of Somatic Embryos and Plant Regeneration
5.6. Types of Suspension Cultures
5.7. Growth Patterns in Suspension Culture
5.8. Measurement of Cell Growth in Suspension Cultures
5.9. Synchronization of Suspension Cell Cultures
5.10. Technique for Single Cell Culture
5.11. Tests for Viability of Cultured Cells
5.12. Advantages of Cell and Suspension Culture
5.13. Plant Cell Reactors
5.14. Immobilized Plant Cell Reactors
5.15. Culture of Isolated Single Cells
6. Somatic Embryogenesis
6.1. Introduction
6.2. Some Examples of Somatic Embryogenesis
6.3. Theories on Embryogenesis
6.4. Factors Affecting Somatic Embryogenesis
6.5. Anatomy and Cytology to Somatic Embryo Development
7. Synthetic Seeds
7.1. Introduction
7.2. Encapsulation
7.3. Production of Artificial Seeds
7.4. Applications
7.5. Limitations
7.6. Uses of Artificial Seeds
7.7. Reference
8. Micropropagation of Higher Plants
8.1. Introduction
8.2. Somatic Cell Embryogenesis
8.3. Production of Adventitious Shoots and Related Organs
8.4. Enhanced Axillary Branching
8.5. Steps Involved in Plant Multiplication
8.6. Buds Culture
8.7. Factors Affecting Micropropagation
8.8. Micropropagation of Ornamental Plants
8.9. Advantages of Micropropagation
9. Haploid Production
9.1. Introduction
9.2. Anther Culture
9.3. Pollen Culture
9.4. Comparison between Anther and Pollen Cultures
9.5. Significance of Uses of Haploids
9.6. Limitations
9.7. Gynogenesis
9.8. Bulbosum Technique
10. Nucellus Culture, Ovule Culture, Ovary Culture and Flower Culture
10.1. Nucellus Culture
10.2. Ovule Culture
10.3. Ovary Culture
10.4. Flower Culture
11. Embryo and Endosperm Culture
11.1. Embryo Culture
11.2. Endosperm Culture
12. Protoplast Culture and Somatic Hybridization
12.1. Introduction
12.2. Sources of Protoplasts
12.3. Isolation of Protoplasts
12.4. Culture of Protoplasts
12.5. Applications
12.6. Protoplast Fusion and Somatic Hybridization
12.7. Cybrids
12.8. Applications
13. Somaclonal Variations
13.1. Introduction
13.2. Origin of Somaclonal Variation
13.3. Isolation of Variants
13.4. Gametoclonal Variation
14. Germplasm Conservation
14.1. Introduction
14.2. Determination of Cell Survival
14.3. Action of Cryoprotectants
14.4. Factors Affecting Revival of Frozen Cells
14.5. Applications
15. Production of Secondary Metabolites Through Tissue Culture
15.1. Introduction
15.2. Types of Cultures
15.3. Bioreactors
15.4. Cell Immobilization
15.5. Improving the Product Quality and Quantity
15.6. Commercial Production
15.7. Characteristics of Plant Cells in Suspension Culture
15.8. Factors Affecting Secondary Metabolite Production in Cell
Suspension Cultures
15.9. Ways to Increase Product Formation
16. Isolation of Mutants From Cell Culture
16.1. Introduction
17. Practicals
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